How to measure rod/spindle shaped particle size distribution from FESEM images?

FESEM image showing dense sindle like geometry. Need to measure the particle size distribution.

22 June 2020 3,136 1 View

What is typical range of Fermi energy in Graphene? and Relation between conductivity and Fermi energy of Grpahene?

Conductivity has an inverse relation with Fermi velocity (VF). But Fermi velocity (VF) is directly proportional to Fermi energy.

18 June 2020 8,140 2 View

Which is the website for eBURST analysis of MLST data?

I have done MLST analysis for my isolates on the pubmlst website. I am trying to do the eBURST analysis for the allelic profiles I got from the MLST analysis. I checked a number of papers for the...

06 February 2020 6,464 3 View

NF Kb estimation: any available biomolecule in serum which can estimate the expression of NF-KB?

nuclear factor kappa light chain enhancer of activated B cells which gives estimate of cytokine release, ultimately inflammation . Is there any diagnostic kit is available which can estimate its...

28 July 2019 1,864 5 View

Can someone suggest what type of microscope would be best for imaging human hematopoietic colonies on MethoCult?

I am currently inducing human pluripotent stem cells towards the hematopoietic lineage and assessing the functional capacity of the progenitors by performing colony based assay on MethoCult. I see...

19 February 2019 1,562 3 View

Will the availability of high speed internet be a major drawback in IoT development and implementation?

This is also a part of my work... So i want more about this in details. Please help me by giving answer of this question in details......... THANK YOU......

10 January 2019 8,102 3 View

Can anyone help me how to solved two nonlinear coupled differential equations using matlab?

I have attached the equations

05 January 2019 7,955 5 View

Has anyone used Lipofectamine LTX + Plus Reagent for transfection in HEK 293Tcells?

Tell me about your experiences in transfection please, thanks!

09 December 2018 8,886 2 View

Does anyone please let me know the standards to perform the mechanical tests (compression behavior) on polymeric scaffolds.??

Please shower your insights on testing parameters of polymeric scaffolds.

29 January 2018 8,834 3 View

Plz can anyone suggest mol biolab in INDIA which is working well on CRISPRcas System in plants wthquality publica'ns & well expertise team?

I want to get trained on latest gene editing technology like CRISPR CaS System on plant genome. Please can anyone suggest molecular biology lab in INDIA which is working well on CRISPR cas System...

20 November 2017 9,450 7 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View