How to get Proteinase K resistant bands of PrPSc from ScN2a lysate by western blot?

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List Plasmid suitable for Homology cassette in CRISPR-Cas9 protocol for Knock-In in mammalian cell line ?

Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...

25 June 2024 1,197 0 View

Why is the charging time so much longer than the discharge time in GCD testing for some materials?

Please explain Why the charging time is more than discharging time in some cases of GCD measurements of electrode in 3-electrode system.

18 April 2024 9,942 2 View

For natural fiber characterization which density should be calculated?

Linear Density or Density of natural fiber

17 April 2024 9,686 0 View

I am not getting sharp band of PCR products. How I can obtain clear PCR bands for my products ?

I'm having trouble obtaining clear PCR bands for DNA fragments ranging from 907 bp to 655 bp. I've tried various methods, including: Testing different brands of Taq DNA polymerase (Takara, NEB,...

12 April 2024 7,845 9 View

What is Heritage Dining? how sustainability is rooted in traditional culinary practices across the globe?

How traditional cooking techniques or heritage culinary practices were the unnoticed sustainable practices that were perfect and can now be used to create more awareness in todays culinary world.

03 April 2024 9,708 1 View

What is sustainable talent management? how to manage staff sustainably in star Hotels?

Understanding sustainable aspect in terms of employee, employer relationship, better workplace environment, equality at workplace etc...

14 December 2023 1,111 1 View

Respected all, Could you kindly inform me which software is commonly used for drawing the anatomy of plants?

Suggests me some softwares. Thank you.

31 July 2023 4,314 1 View

Respected all, please tell me which software is being used to draw the anatomy of plants. How can I draw these diagrams graphically?

Suggests me some softwares. Thank you.

31 July 2023 3,392 1 View

Hi, Can anyone please suggest is it better to use methanol/acetone for fixation instead of PFA for immunfluorescence for 53BP1 (DNA damage marker)?

When i tried fixing cells with methanol/acetone , i started getting signals which previously i was not able to( i thought my primary antibody is not working). Also signals were brighter in...

24 July 2023 4,122 0 View

What is the difference between inta-nigral and intra-striatum administration of 6-OHDA as a PD rat model?

11 June 2023 9,090 1 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View