Hi, Can anyone please suggest is it better to use methanol/acetone for fixation instead of PFA for immunfluorescence for 53BP1 (DNA damage marker)?

25 July 2023 0 4K Report

When i tried fixing cells with methanol/acetone , i started getting signals which previously i was not able to( i thought my primary antibody is not working). Also signals were brighter in intensity when compared to positive control (where cells were fixed with PFA). But i am not able to figureout is it because of the change in fixative

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