I want to disrupt Gram + ve cell to get intracellular extract which is having an enzyme. This enzyme should remain functional after cell disruption.

I am using ultrasonicator (20 K hz, 30 pulses for 30 min at 100 W) for it, but i didnot get cell disruption. (this sonicator has max limit of 20 K hz frequency) ,what can i do for getting cell free extract by using same sonicator.

Thank you.

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