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In the study, 5 Bacteria (A,B,C,D and E) are determined for growth at various temperature (10,20,30,40 and 50 degree c.) ,different pH (5,6, 7, 8 and 9) and agitation (0, 50,100,150 and 200 rpm)....
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I want to disrupt Gram + ve cell to get intracellular extract which is having an enzyme. This enzyme should remain functional after cell disruption. I am using ultrasonicator (20 K hz, 30 pulses...
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