How to get a high signal of Na+K+ ATPase in endothelial cells isolated from corners using immunofluorescence staining?
I am interested in showing a high signal of Na+K+ ATPase in endothelial cells isolated from corneas and in B4G12 cell line. Thus, I appreciate getting from you a detailed protocol showing the Na-K ATPase using immunofluorescence staining.
you might need to use Anti-Sodium Potassium ATPase antibody (ab58475) ( they stain total Na-K ATPase protein). If you know what isoform (alpha 1?) of Na-K ATPase is predominantly present in the corneal endothelial cells of your interest than you might need to use more specific Abs. Abcam seems to have all of the required Abs. The protocols for corneal endothelial cell staining you can find in these articles (attached)
Thank you for all your help i really appreciate it. I was having some issues to get it working. I will follow your recommendations and hopefully it will work.