The GeneX is lethal in homozygous state. I crossed GeneX/Cyo x GeneX/Cyo only to get adult non CyO flies post drug treatment to reverse the GenX function. How to ascertain these flies are GenX homozygotes and without the Cyo balancer. I want to do a PCR with GeneX and CyO primer sets. But i cannot find CyO nucleotide sequence.
If your blancer has GFP or RFP (such as actin-GFP), you can do PCR with GFP or RFP specific primer.
Dear Hitoshi, I do not have a marker on the balancer chromosome. Is there any other way to identify the presence of Cyo Balancer?
Yes, if you can use GFP dissection scope, it should be easy.
If you want to see homozygote in adults, I think CyO Dfd-YFP would be best choice.
See the following link from Bloomington.
http://flystocks.bio.indiana.edu/Browse/GFP/GFP_fluorBal.php
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