Gargi Nanda

6 Questions 15 Answers 0 Followers

Questions related from Gargi Nanda

How to re-develop an EMSA blot?

Hi all, Is it possible to redevelop an EMSA blot which has already been exposed? I am using ThermoScientific LightShift Chemiluminescence kit and believe that my blot could be improved if I had...

04 April 2017 3,944 2 View

How to genetically ascertain that the flies are Cyo?

The GeneX is lethal in homozygous state. I crossed GeneX/Cyo x GeneX/Cyo only to get adult non CyO flies post drug treatment to reverse the GenX function. How to ascertain these flies are GenX...

09 September 2016 8,247 3 View

Does filter sterilization of FBS affect it by devoid its hydrophobic lipid constituents?

I usually filter sterilise the media w/ FBS or FBS alone (post heat inactivation). I use 0.22um PVDA membrane filters or syringe filters at times. I also use sensitive cell lines. The cells lines...

04 April 2015 3,716 3 View

How many nucleotides upstream and downstream should I include to clone a minigene?

I intend to analyse the effect of an unstable intron element in exon splicing. For this, I'm planning to include the flanking exons (say 3rd and 4th exons of a gene) by PCR amplifying them from...

11 November 2014 7,951 3 View

How can I best carry out RNA extraction from corneal tissues?

I'm facing problems regarding extraction of RNA from corneal tissues acquired post DSEK. I have been using Qiagen RNA extraction Kit so far. But the conc. is as low as 2ng/ul with 260/280 2.23...

09 September 2014 9,756 8 View

Can you troubleshoot my Simple Allele-discriminating PCR (SAP)?

I'm trying to perform SAP for a particular SNP using usual Taq pol (not hifid) and primers designed using WASP (http://bioinfo.biotec.or.th/WASP); as suggested by the authors (Bui and Liu). But...

09 September 2014 2,425 10 View