I am trying to generate a gene knockdown in THP1 cells. I could successfully do the transduction however , i am not able to generate the single cell clones of THP1. I prepared the serial diluation and seeded single cell in 96 well flat bottom plate. I added conditioned media and also used Rock inhibitor. The single cells are not propagating at all and rather , the single cell didnot survive. Can anyone help or could give some advice ?