Hi, I am currently working with AuNPs and I want to know if there is any possibility to coat the surface of AuNPs (14nm) with PLL (Poly-L-lysine), there is two types of PLL:
If anyone have some information I would be grateful if you can help me to achieve this ligand exchange, I tried it but after the addition of PLL hydrobromide my AuNPs showed a blue coloration (aggregation).
Thank you in advance
Did you think of encapsulation techniques?
By double emulsion solvent evaporation for example
So that the Poly Lysine polymer becomes a nanoparticle containing AU NPs inside them
Actually my idea is a coating approach in order to replace the citrate groups of AuNPs with PPL, its is possible just by some encubation method or I need a cross linker technique to achieve it
You may find this article helpful
https://www.researchgate.net/profile/Li_Niu7/publication/26826766_Water-Soluble_Graphene_Covalently_Functionalized_by_Biocompatible_Poly-L-lysine/links/5520f4910cf2a2d9e1434eef.pdf
Article Water-Soluble Graphene Covalently Functionalized by Biocompa...
Hi Carlos,
You can initially functionalise AuNPs with thiloated PEG having COOH group at the other end. SH-PEG-COOH is the molecule you can add to the citrate-AuNP. After ligand exchange, you can activate the COOH groups using EDC/NHS chemistry. After activation it can easily conjugate to NH2 groups of polylysine.
Good Luck
Thank you so much for your time and help I also found a paper where some scientist bind PLL to AuNPs surface by electrostatic interaction
Hi Carlos,
Why not save yourself the time and effort required to carry our these experiments and purchase AuNP's already functionalised with PLL. The time and money saved easily outweighs the cost of these materials.
BBI Solutions offer such products as part of our particle range. They are available to purchase direct from their website in 0.25ml and 1ml vials.
http://www.bbisolutions.com/products/1081-ua-conjugate-cationic-colloidal-gold-15nm.
Larger volumes could be purchased upon request
Please let me know if you have any further questions - id be happy to continue this conversation
Kind regards
Darren
hi everyone,
Sorry i haven't an answer for your question because in my turn i need a method to synthesize AuNPs with different sizes directly in organic solvent (chlorobenzene, chloroform or toluene) or a method to dry them completly and redissolve them in chlorobenzene or chloroform without aggregation
i'll be gratefull if u can help
best regards