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Questions related from Gangula Abilash
Hello, I am trying to isolate cell membrane from a human cancer cell line. At the end of the procedure, I am not sure whether I got the cell membrane. BCA assay did not show any color. Below is...
16 June 2023 5,711 4 View
Hi, I am trying to develop a competitive lateral flow assay to detect a protein. I coat the protein on the test line and use gold nanoparticle-antibody as the conjugate that binds to the test...
15 February 2019 2,188 4 View
Hi, I am trying to synthesize ultra small 2nm gold nanoparticles by NaBH4 reduction (Brust Method). What should be the characteristic UV-Vis spectrum of 2nm size gold nanoparticles. Should we or...
19 September 2018 4,498 9 View
Hi, I am trying to use lateral flow assay to detect the whole intact virus by targeting the envelop proteins. That is, I use antibodies against the envelop protein of the virus for my assay. I...
19 April 2018 3,444 6 View
We are trying to detect a Virus using a sandwich lateral flow assay. We are targeting the epitopes on the envelop protein of the virus. Is there anyway I can inactivate the disinfect the virus,...
16 March 2018 2,669 3 View
I am trying to develop a gold nanoparticle based lateral flow sensor to detect a whole intact Virus. I have tried with 3 different antibodies of the virus. But, I was not successful. Reading the...
09 February 2018 3,643 8 View
Hi, I request your valuable suggestions. I was trying to do AuNP based Lateral Flow Immuno Assay LFIA to detect Virus. I have 3 different antibodies for this virus. I was trying to do a sandwich...
05 December 2017 9,534 11 View
Generally EDC-sulfo NHS activation is done for carboxylic acid moeties on a nanoparticle surface or a planar substrate. Can we do EDC-Sulfo NHS activation for small molecules of around 300...
01 December 2017 9,208 2 View
Hi Everyone, I need to activate COOH groups of COOH-PEG-SH and further conjugate activated COOH groups with amine of an antibody. I want thiol groups to be intact and functional for further...
10 January 2017 7,539 1 View
I would like to know whether ammonium per sulfate (APS) is a good radical initiator for synthesizing PMMA nanoparticles by emulsion radical polymerisation. Or, does it have any drawbacks...
05 October 2016 7,845 8 View
Can anyone who worked with quantum dots suggest what be the ideal quantum dot to use for paper based lateral flow assay. I should be able to conjugate antibody to it and we are looking for a...
18 August 2016 7,671 3 View
I need to develop a portable low cost sensor using a nitrocellulose membrane (NM). I have done an assay for my analyte on a 96 well plate using monoclonal and polyclonol antibodies and a labeling...
01 June 2016 9,684 1 View
I have to immobilize an antibody onto a paper (preferably nitrocellulose) to develop a paper based immunosensor. Can anyone suggest a reliable and reproducible method of doing so without loosing...
31 May 2016 2,931 4 View
I would like to capture guanosine, a nucleoside, from a sample. Based on the specific H-bonding interaction between nucleobases cytosine and guanine, I would like to design a sequence of...
09 May 2016 7,205 4 View
In a UV-vis apectrophotometer, what does it mean if the absorption spectrum is coming in the negative region of the y-axis (absorbance) even after taking a baseline with reference?
26 December 2013 3,898 21 View
I am trying to do a gold nanoparticle (AuNP) based lateral flow assay for detecting an analyte. It is a competitive assay, where in the conjugate pad has the AuNP-Antibody conjugate, the...
01 January 1970 8,013 12 View
I have to develop a paper strip for detection of a small molecule, a nucleoside. I need some suggestions on the arrangement of different pads as I am doing the paper based sensor for the first...
01 January 1970 4,447 7 View
