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Questions related from Gangula Abilash
Hello, I am trying to isolate cell membrane from a human cancer cell line. At the end of the procedure, I am not sure whether I got the cell membrane. BCA assay did not show any color. Below is...
16 June 2023 5,779 4 View
Hi, I am trying to develop a competitive lateral flow assay to detect a protein. I coat the protein on the test line and use gold nanoparticle-antibody as the conjugate that binds to the test...
15 February 2019 2,239 4 View
Hi, I am trying to synthesize ultra small 2nm gold nanoparticles by NaBH4 reduction (Brust Method). What should be the characteristic UV-Vis spectrum of 2nm size gold nanoparticles. Should we or...
19 September 2018 4,558 9 View
Hi, I am trying to use lateral flow assay to detect the whole intact virus by targeting the envelop proteins. That is, I use antibodies against the envelop protein of the virus for my assay. I...
19 April 2018 3,504 6 View
We are trying to detect a Virus using a sandwich lateral flow assay. We are targeting the epitopes on the envelop protein of the virus. Is there anyway I can inactivate the disinfect the virus,...
16 March 2018 2,734 3 View
I am trying to develop a gold nanoparticle based lateral flow sensor to detect a whole intact Virus. I have tried with 3 different antibodies of the virus. But, I was not successful. Reading the...
09 February 2018 3,702 8 View
Hi, I request your valuable suggestions. I was trying to do AuNP based Lateral Flow Immuno Assay LFIA to detect Virus. I have 3 different antibodies for this virus. I was trying to do a sandwich...
05 December 2017 9,581 11 View
Generally EDC-sulfo NHS activation is done for carboxylic acid moeties on a nanoparticle surface or a planar substrate. Can we do EDC-Sulfo NHS activation for small molecules of around 300...
01 December 2017 9,274 2 View
Hello, We work extensively on conjugating small molecules (like crosslinking agents, etc) to proteins and peptides. Hence, we often face with the task of separating the unreacted reactants and...
21 February 2017 1,651 4 View
Hi Everyone, I need to activate COOH groups of COOH-PEG-SH and further conjugate activated COOH groups with amine of an antibody. I want thiol groups to be intact and functional for further...
10 January 2017 7,574 1 View
My purpose is to achieve more thiol (-SH) groups in an antibody without loosing its antigen recognizing property. So, we would like to cleave the disulphide bond at the hinge region which is...
23 December 2016 5,446 2 View
I would like to know whether ammonium per sulfate (APS) is a good radical initiator for synthesizing PMMA nanoparticles by emulsion radical polymerisation. Or, does it have any drawbacks...
05 October 2016 7,915 8 View
I need to develop a paper based assay to determine urinary creatinine levels. Since creatinine is a small molecule, I need to conjugate it to a protein like BSA to make it suitable for the...
19 September 2016 8,118 1 View
Can anyone who worked with quantum dots suggest what be the ideal quantum dot to use for paper based lateral flow assay. I should be able to conjugate antibody to it and we are looking for a...
18 August 2016 7,715 3 View
I need to increase the sensitivity of a paper based assay so that even trace amounts of antigen immobilized on nitrocellulose membrane would be able to capture the gold nanoparticle-antibody...
15 August 2016 4,108 1 View
I have coated anti-human secondary antibody on a nitro cellulose paper. I have dipped this paper into a solution of gold nanoparticles-human antibody conjugate. Will the gold nanoparticle-primary...
16 June 2016 1,543 12 View
I need to develop a portable low cost sensor using a nitrocellulose membrane (NM). I have done an assay for my analyte on a 96 well plate using monoclonal and polyclonol antibodies and a labeling...
01 June 2016 9,719 1 View
I have to immobilize an antibody onto a paper (preferably nitrocellulose) to develop a paper based immunosensor. Can anyone suggest a reliable and reproducible method of doing so without loosing...
31 May 2016 2,984 4 View
I would like to capture guanosine, a nucleoside, from a sample. Based on the specific H-bonding interaction between nucleobases cytosine and guanine, I would like to design a sequence of...
09 May 2016 7,251 4 View
In a UV-vis apectrophotometer, what does it mean if the absorption spectrum is coming in the negative region of the y-axis (absorbance) even after taking a baseline with reference?
26 December 2013 3,955 21 View
In the catalysed reduction of 4-nitrophenol to 4-aminophenol, why is the increase in the absorbance of 4-aminophenol not as significant as the decrease in the absorbance of 4-nitrophenol? In...
26 December 2013 7,735 2 View
Surface plasmon resonance (SPR) is an important phenomena because of which Au and Ag NP exhibit interesting optical properties that are sensitive to size, shape, and dielectric constant. Does...
12 June 2013 9,439 7 View
I am trying to do a gold nanoparticle (AuNP) based lateral flow assay for detecting an analyte. It is a competitive assay, where in the conjugate pad has the AuNP-Antibody conjugate, the...
01 January 1970 8,082 12 View
I have to develop a paper strip for detection of a small molecule, a nucleoside. I need some suggestions on the arrangement of different pads as I am doing the paper based sensor for the first...
01 January 1970 4,493 7 View
