Trichoderma is well known as an effective biocontrol agent, used to control soilborne pathogens and some foliar disease.
Hello, after a rapid investigation for you i fined this description tha can be applicable for biocontrol agents like Trichoderma, Pseudomonas....
Wettable powder formulations of herbicides:
A formulation comprising: (a) at least one herbicide, (b) at least one porous solid carrier material, and (c) a nonionic surfactant, which formulation contains 30 to 60 % by weight, based on the total formulation, of at least one nonionic surfactant of the formula (I): R1-(O-R2-)nO-R3, wherein R1 is C8-C16alkyl or C8-C16alkenyl, R2 is identical or different C2-C4 alkylene, R3 is hydrogen or C1-C4alkyl, and n is an integer from 5-10. High biological activity is achieved with the solid formulation at low rates of application.
NB: our team will try these formullation for our effective biocontrol agents belonging to the genus Pseudomonas described in Amkraz et al. 2010 and Amkraz 2013.
Sincerly yours,
Dr. Amkraz Nadiya
Follow general formulation guideline , considering Trichoderma spores/ biomass as active ingredient you can develop WDP and liquid formulation of Trichoderma. Be careful in selecting additives and carrier which shouldn;'t have any adverse impact on spore viability and shelf life , carry out weekly germination test ....
We have had success when Trichoderma was grown on peat-bran mixture.
After drying, it is easy to apply.
The spores can also be coated on seeds, we used radish seeds which are really tiny, and it worked well.
A wheat bran/peat mixture for growing and applying Trichoderma is fairly easy to use when dry, but can be more difficult to work with if you want to wet it. For this and some other types of carriers, see for example: Field Control of Cotton Seedling Diseases with Trichoderma Virens/chemical Seed Treatments by Howell et al. (1997).
There have been several studies using alginate prills that might be an option for you (e.g. http://download.springer.com/static/pdf/785/art%253A10.1023%252FA%253A1008862326518.pdf?auth66=1404564863_8216b482e9e06f5b174b6a01be8f5fba&ext=.pdf; DOI: 10.1080/09583159650039368; DOI: 10.1139/cjm-46-8-730, etc.)
Dear Mr. Mohammad Ibrahium Al-Masri,
We know that spore viability most important factor for Trichoderma formulation.Liquid & solid substrate are use for formulation. Solid substrate most effective than liquid culture & in future long term retain spore.Corn grain powder, Peanut shell powder produce more spore in solid culture if maintain appropriate moisture.In future long term preserve you can add CMC, talc powder, Tween 80, glycerol etc in the solid culture.
Best Regards
Rabiul Islam
69
Hi Mohammad
Many useful papers for you on attachment 1
https://www.tandfonline.com/doi/full/10.1080/03235400500094720?src=recsys
6
https://www.agrilife.in/pdf/biopesticides/biopesti_micro/dp/ecosom-%20TH_dp.pdf
7
https://agritech.tnau.ac.in/crop_protection/crop_prot_biocontrol%20control_agents.html
8
http://www.ppjonline.org/journal/download_pdf.php?spage=283&volume=22&number=3
9
Article A new oil-based formulation of Trichoderma asperellum for th...
13
https://www.omicsonline.org/open-access/liquid-formulation-of-trichoderma-species-for-management-of-gray-mold-incastor-ricinus-communis-l-and-alternaria-leaf-blight-in-sunflowerhelianthus-annuus-l-2155-6202-1000149.php?aid=45252
16
https://www3.epa.gov/pesticides/chem_search/reg_actions/registration/decision_PC-119208_4-Mar-10.pdf
18
http://www.bfgsupply.com/_CE/pagecontent/Documents/2016_Horticultural/BFG_2016_17_Hort_8_Organics.pdf
19
https://agris.fao.org/agris-search/search.do?recordID=US201500075674
20
https://www.indianspicesociety.in/josac/index.php/josac/article/viewFile/381/318
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