ANSYS FLUENT is only launching in parallel. Can somebody please help?

My fluent is not launching in series at all, even when I uncheck the parallel option and i also have no option for using series. Please help!!! Many thanks!

04 September 2020 4,199 4 View

I am trying to simulate an electric arc formed because of current flowing through air between 2 conductors in ANSYS Fluent. Can i use MHD module?

If i use the MHD module, i will have to provide the values of external magnetic field, but isn't the magnetic field established as a consequence of the current flowing through the air? How am i...

15 August 2020 200 1 View

Removal of dead cells before harvesting for Proteomics?

Dear all, I am culturing some suspension cell lines. I am planning to perform proteomics (LC-MS/MS) experiments on them. Unlike adherent cells (viable cells are adhering to the substrate and...

03 March 2020 2,329 3 View

Enrichment of AHA-tagged proteins: Should I use DBCO- or Alkyne-based methods?

Dear colleagues, I would like to seek your kind advice on click chemistry enrichment of azidohomoalanine (AGA)-tagged proteins. From literature, I learned that there are 2 main avenues, either...

18 February 2020 8,725 2 View

Why do you need to preheat antigen retrieval buffer?

I'm troubleshooting immunofluorescence staining for some antibodies. Some work fine with my protocol [H2AX, Ki67, 3-nitrotyrosine], some stained once and never again [8oxoG], and some won't stain...

21 April 2019 5,510 3 View

What is the ideal activation time for stain-free gels?

From Bio-Rad's user guide (see below) it is not clear to me what is the best activation time to use. It states that 1 min gives optimal results for WB followed by immunodetection, but that 5 mins...

25 March 2019 1,508 4 View

Who knows a good surgical monitoring system for mice?

I am looking for a good surgical monitoring system for mice, mainly ECG recording. For different suppliers it is mostly the same Scintica Rodent Surgical Monitor+ system. Does anyone know a...

02 March 2019 5,069 1 View

Who knows a good surgical monitoring system for mice?

I am looking for a good surgical monitoring system for mice, mainly ECG recording. For different suppliers it is mostly the same Scintica Rodent Surgical Monitor+ system. Does anyone know a...

28 February 2019 2,000 1 View

What is the composition of Vector M.O.M. Blocking Reagent?

Has anybody an idea what might be in the Vector M.O.M. blocking reagent? The kit works very well in background reduction and one could use rabbit antibodies on rabbit etc. if we would know the...

11 December 2018 7,269 3 View

What is the composition of Vector M.O.M. Blocking Reagent?

Has anybody an idea what might be in the Vector M.O.M. blocking reagent? The kit works very well in background reduction and one could use rabbit antibodies on rabbit etc. if we would know the...

02 December 2018 7,486 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

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03 March 2021 5,360 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View