Murine protein expressed in HEK293 cells used for aspirations in mouse model ?

I am planning to express a murine protein X with a Myc tag in HEK293F cell line - purify the protein - check the purity of the protein - use that protein - X with Myc tag to aspirate mouse. Thus,...

12 February 2020 4,483 1 View

How to get an Asymptote from multiple coordinates obtained from Machine Learning ?

I want know about an algorithm or formula to find the asymptote from coordinates obtained from Machine Learning. Like the ML will always give points precise & closer to the Asymptote if I ran it,...

11 October 2019 1,025 2 View

I have completed my bachelor's in Electrical Engineering and I am looking forward to start learning more about smart grid .From where should I start.?

I want to learn about smart grid and simulating a smart grid network and also the data analytics used in smart grid network .

23 May 2018 6,165 3 View

Has Any One Tried 365nm Fixed Wavelength LED 10 Watts?

29 September 2016 8,339 3 View

Does anyone have experience working with Injectable Cyanocobalamin in rats?

15 September 2014 1,045 4 View

When I tried to precipitate my protein with acetone or TCA, it did not precipitate. But when I mix it with water it precipitated - any thoughts?

My protein is acid extracted. I need to know the mechanism behind it.

29 May 2014 6,787 3 View

Free software to conduct an online quiz?

From the last few months, I am trying to generate an online Quiz for my student, I have prepared some of them also, but find two difficulties: 1. Some students can't open the exam in their...

24 December 2013 2,307 3 View

Shortcut key to merge some cells in table & to split the same!

Can anybody suggest the shortcut keys for Merging & splitting the cell in MS word & MS Excel?

05 October 2013 9,215 13 View

What is the ideal dilution for using the Primary and Secondary Antibody Concentration for 6x HIS tag : Protein?

The 1:2000 for primary and 1:20000 for secondary gives a very dark background .

22 May 2013 4,813 3 View

What if the restriction digest pattern of plasmid midiprep varies from the same plasmid's miniprep?

I normally inoculate 5 - 7 colonies from selective antibiotic LB plate after transformation in the liquid LB with the same concentration of the same antibiotic. The next day, I do plasmid miniprep...

27 February 2013 4,896 12 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What are the potential shell well and atomic solvation parameters for the boron atom?

I have a protein-ligand docking project. The ligand contains boron atoms that are not detected by Autodock software. To solve this problem, it is necessary to introduce the boron atom to...

19 February 2021 1,236 1 View

Can we incorporate unnatural amino acids into protein using software for graphical image purpose?

I am looking to incorporate a unnatural amino acids which is not available in the swisssidechain database into a protein crystal structure of pdb? I am using chimera 1.14 vis. tool?

26 January 2021 4,064 3 View

How do you get around this AutoDock Vina error?

I get the following error messages after running the simulation: Sorry, there are no Gasteiger parameters available for atom ligandx.ligand:A:lig1:O7 AutoDock Vina ligand docking initiated for...

26 January 2021 3,822 3 View

How to generate .dlg files on PyRx?

Hi. I'm using the PyRx version 0.8 and getting the error message: WARNING: Filename mismatch: "2DFN_ADP_CL.maps.fld" :: "..\2DFN_ADP_CL.maps.fld" C:\Program Files (x86)\The Scripps Research...

17 January 2021 843 3 View

How to phosphorylate specific residues on pymol?

Hi everyone, I am using the pyTMs plug in on Pymol to phosphorylate a particular threonine in my protein. I am having difficulty in selecting only one or two residues - it seems I can only...

05 January 2021 230 3 View

Is there any online webserver for DNA-ligand docking?

I have used Autodock 4.2 for docking studies and trying haddock for DNA-Ligand docking. It shows an error and not supported DNA PDB file. Suggest a template for DNA and ligand PDB file and also...

29 December 2020 1,322 8 View

How to interpret Energy minimization results?

I have conducted an EM using YASARA server, and the output PDB is viewed in PyMol to understand the conformational changes between model that is energy minimized and the original model. I have...

28 December 2020 2,029 2 View

Autodock Raccoon - the following maps are missing?

I've generated a set of maps for my receptor using ADT (AutoDockTools). It has resulted in the files: .A.map .C.map .HD.map .N.map .NA.map .OA.map .SA.map .d.map .e.map But when I try loading the...

20 December 2020 7,721 2 View