Dear Xi Gong ,

You might want to use this tool from ExPASy:

https://web.expasy.org/compute_pi/

There you can add your UniProt accessions in a list (separated by Tabs, or new lines, or even spaces) and it will give you the output pI (Isoelectric point).

For example:

Q9H9K5

P04439

P08246

The output will be:

MER34_HUMAN (Q9H9K5):

• CHAIN 27-563 Endogenous retroviral envelope protein HEMO Theoretical pI: 8.18 Molecular weight (average): 60691.58
• CHAIN 27-432 Endogenous retroviral envelope protein HEMO, secreted form Theoretical pI: 6.58 Molecular weight (average): 45691.16

HLAA_HUMAN (P04439):

• CHAIN 25-365 HLA class I histocompatibility antigen, A alpha chain Theoretical pI: 5.55 Molecular weight (average): 38316.61

ELNE_HUMAN (P08246):

• CHAIN 30-267 Neutrophil elastase Theoretical pI: 9.89 Molecular weight (average): 25561.40

I hope this is what you were looking for. Best regards,

Julia