I want to determine the Effective permeability (Peff) for the bioactive through insitu small intestinal perfusion study. I read the articles related to this method. Researchers are using this method to determine the Peff for the drugs. So they dilute the drug with buffer and they are passing through the intestine. The bioactive I'm using is lipid soluble, so I'm using oil-water emulsion and the emulsifier is protein. It has to be digested in the stomach phase. How could I proceed. I think I can't take it the emulsion straight for the intestinal perfusion study. How could I incorporate the digestion step before the intestine?
Please help..
Effective permeability refers to permeability when one fluid flows in the presence of a second fluid in a pore system. It occurs when permeability exhibited by a porous medium to one fluid phase when there is physical interaction between this phase and other fluid phases. Use alkali and water mixture or use a protein transporter for it. You can also vitamin B6 as transporter. Absolute permeability: is the permeability of a porous medium saturated with a single fluid (e.g. Sw=1) Absolute permeability can be calculated from the steady-state flow equation (1D, Linear Flow; Darcy Units):Commonly, reservoirs contain 2 or 3 fluids Water-oil systems, Oil-gas systems, Water-gas systems,Three phase systems (water, oil, and gas)To evaluate multiphase systems, must consider the effective and relative permeability. Because vitamins also absorb in the stomach therefore, it will be a better transporter for your purpose,
Correct dilution technique and effective permeability is very important to save the concentration of a substance that enters the body. Only in this case it is possible to compare data from different experiments.
I hope some papers will help you.
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