We can arrange our lab setup according to the APHA 21st ed; but we can not find those species (without Daphnia) described in Sri Lanka, can we use zebrafish (Danio rerio) - freshwater fish for this?
Is is not that difficult to purchase Daphnia magna specially for ecotoxicity testing and maintain a culture of in the laboratory.There is a kit for Daphnia tests that used the winter-eggs of Daphnia magna as the source of the test animals. If you keep a few animals from such a kid and keep and feed them according to the standard you will have always have animals for testing.
Fish have a quite different sensitivity to chemicals compared to crustaceans so replacing Daphnia with zebrafish does not give a similar results. Generally you should not expect that regulators will accept that you replace tests that are this different.
Since you have "seawater based drilling mud" it is likely that the salt will have effect by itself and additionally I suspect that the intension is to release the mud to the sea. Therefore you really should base your testing on marine species. Perhaps a copepod like Acartia tonsa, which can be cultures.
Dear Dr. Henrik Andersen,
thank you for the reply, I tried to carryout with marine species (according to the APHA). but it was really difficult to keep them alive even in the storage tank. in your explanation I figure, that we can not use Daphnia also for this sea water based samples. therefore Acartia tonsa (copepoda) is the correct specie for this, Am I correct?
thank you!!!
AmilA.
Acartia tonsa is just one of the possibilities.
It has been used for evaluataing toxicity in materials related to the oil exploration in the North Sea. There is a published standard for acute test: "Acute toxicity to the crustacean Acartia tonsa, ISO 14669" and a chronic test "Larval Development Test, Acartia tonsa ISO Draft (2011)".
Dear Sir,
thank you very much for your attention for this, really appreciated!!!!! I could find, your recommended tests in this file which is attached. I going give my attention for these methods. I'm really expect your kind help regarding this in future!!
best regards!!!
AmilA.
There are a range of possible marine species that could be used - both water and sediment. Maybe Tisbe battagliai would be a better choice than Acartia....they are much easier to culture and handle....or how about an embryo-larval test with mussels or oysters? I can provide references if required?
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