How much is the role of education in living a happy life?

What is the purpose of education and what we get from education?

23 September 2020 5,949 4 View

What should be the standard glucose concentration to be used in high glucose studies, that match the physiological levels?

Forgive me for being Naive. I have read that the postprandial concentration normally doesn't go above 140mg/dl, anything above it is considered diabetic. for instance:...

23 July 2019 1,969 4 View

What could be the possible reasons behind the cells dying in the culture plate in few wells while surviving in other, media conditions being same?

I work on MC3T3-E1 cell lines. The cells appear absolutely fine in flasks, but when I seed them in 12/24 well plates for mineralization (nunc thermofischer plates), then cells appear to die in few...

03 February 2019 1,648 3 View

How to extract particular section from text data using NLP in Python?

I have lot of CV (text documents). From these data I want to extract particular section of 'Education Qualification', 'Experience', etc.

03 January 2019 4,454 12 View

Is Nested PCR approach to detect HPV infection in fresh as well as FFPE specimens right?

Hi! I am using nested PCR approach to detect mucosal HPV in cervical specimens as well as from FFPE tissues. This is to increase the sensitivity. But in doing so, I am getting a same similar size...

28 March 2018 9,631 4 View

How to prepare drug library?

There is no known inhibitor for my protein, so i want to screen some drugs which are natural compounds and most probably commercially available, so for the drug library, which database i used to...

03 October 2017 505 2 View

Pyruvate kinase M2 assay?

We have synthesized some inhibitors for PKM2 enzyme. we are planning to perform enzyme kinetic studies. we studied some protocols for the same but conc. of enzymes to be used are missing. some of...

18 September 2017 433 4 View

PLKO1 vectors transduction?

We are going to use SHC001 Scramble vector from sigma it does not contain any shRNA or GFP tag, however, it has ampicillin and puromycin resistance genes. In this case, how to characterize that...

12 September 2017 7,703 6 View

Which form of sh RNA is best for experimental purposes?

20 July 2017 4,414 3 View

Cancer stem cell isolation protocol?

Hello, I am isolation cancer stem cells from MCF-7 cells. I saw protocols for 96 well plate where 1 cell per well is seeded. How many cells should be seeded in 6 well plate.

29 March 2017 5,174 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

What are the potential shell well and atomic solvation parameters for the boron atom?

I have a protein-ligand docking project. The ligand contains boron atoms that are not detected by Autodock software. To solve this problem, it is necessary to introduce the boron atom to...

19 February 2021 1,236 1 View

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19 February 2021 8,647 3 View

How to improve dissolution of neosome?

I have made neosome from herbal drug. Neosomes are stable. But I face problems in dissolution. I used minimum ratio of 1:1:0.25 ( Drug:span 60: Cholesterol and GMs. Now how can I active better...

17 February 2021 3,584 1 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View