09 October 2018 1 5K Report

I would like to extract placenta of rats on E7 in order to perform qPCR, but I don't know how to dissect the tissue properly.

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How can I find Qiime2 bibliographic sources of updated commands?

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It is technically correct to meta-analyse antioxidant capacity results from different techniques?

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How can I compare two kinetic curves?

Hey! I'm looking for ways to compare two kinetic curves over time. My data is composed of neutralizing antibody results during 11 points (vaccine kinetics) in each group (control and...

29 February 2024 9,979 3 View

How can I transform the median to mean when I only have IQR (only Q3-Q1, not the exact values for each), median and sample size?

I'm doing a meta-analysis for continous outcomes and most of the data from the studies is reported as median, IQR (as difference Q3-Q1), I already know Wan (2014) method and McGrath (2021) method,...

13 January 2024 3,397 5 View

When should we update a validated scale or test?

Hello, We designed a series of tests that aim to assess knowledge in first-year university students, and went through the process of validating each one of them (construct validity, through EFA,...

01 January 2024 4,251 4 View

How to convert Shimadzu GC-MS files (.CDF or .qgd) to Agilent MassHunter files (.d)?

Hello everyone, I'm currently working with chromatograms on a Shimadzu system and am seeking assistance in converting files saved in either .CDF or .qgd formats to .d format for use in Mass...

12 December 2023 5,663 2 View

Storage of boiled cell lysates with 2xLaemmli buffer?

I am currently in the process of doing a Western Blot with mESCs after an AHA Assay. I added 25uL of 2xLaemmli buffer and 25uL of AHA buffer (50mM Tris HCL) to my pellet. I usually denature my...

05 December 2023 4,334 3 View

No signal in my Western Blot, after reincubate?

Hi everyone! I am new in lab and I have been having problems with Western Blot, I use a Chemidoc and when I reveal I see nothing, after reincubate, or incubating with a new antibody, the signal is...

29 August 2023 9,130 3 View

Can you pre-fill data on REDCap in a longitudinal study so a participant can edit their last response?

The question is related to a medication list, which I have embedded into a table. Current medications are asked at multiple follow-up points; however, I want participants to have the table...

22 August 2023 8,602 1 View

¿How do we reconstitute thrombin for 3D hydrogels?

Hello everyone. We are using thrombin from bovine plasma (T4648-1KU, sigma). ¿How can we make a stock of 100 U/ml thrombin (0.1% BSA solution)? ¿Should we weigh the powder and reconstitute it...

05 July 2023 2,691 2 View

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