I would like to extract placenta of rats on E7 in order to perform qPCR, but I don't know how to dissect the tissue properly.
Hi Mohamed,
The problem is that on E7 the embrios are too small to dissect this way. I can barely identify the placenta and fetus. Do you have any hints on how to perform it with only 7 days of gestation?
Thank you!
I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...
26 February 2021 3,033 9 View
I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...
10 February 2021 2,305 3 View
Hello! I need to simulate and investigate possible chemical interactions between some polymers and dye molecules. Is there any free reliable software you would recommend? Thank you in advance....
29 January 2021 541 2 View
Hello I have tried to download the new version of the worldclim bioclimatic layers in the R console, since this way I can download spatial subsets. Does anyone have a code that works with the new...
10 September 2020 1,948 5 View
Hi. I am doing factor analysis on a dataset of children responses on a phonological awareness construct. I have 163 items coming from 8 different tasks that measure the same construct. FA parallel...
06 August 2020 7,828 15 View
Hello, I'm currently trying to perform a supervised classification of land cover for remote sensing images. In order to do that, I must create a training imput where I classify a fraction of the...
01 May 2020 7,696 3 View
Hi! I am trying to install R extension on my SPSS 26 for Mac. I already downloaded R (version 3.6, as previous versions are not supported by Catalina). I looked for the R36 extension on the...
17 March 2020 174 5 View
I recently read an article regarding the use of melatonin as a neuroprotective agent using acute ethanol exposure for 1 day and it had good results, I want to know if exists an article using...
03 January 2020 9,709 2 View
I've isolated exosomes from 5ml of cell culture media using ExoQuick TC PLUS kit. I got a visible pellet that was resuspended in the "resuspension buffer" and then purified with the...
13 June 2018 5,514 4 View
I tried with opendrop but the program doesn't work, I'm also trying to make a code for MATLAB, but it has some problemas.
03 November 2017 1,671 5 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...
24 February 2021 9,210 1 View
I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...
24 February 2021 3,623 3 View
Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...
22 February 2021 592 1 View