I have limited amount of samples, so I am trying to get RNA, DNA and protein from the same samples. I used Trizol reagent for RNA isolation, then use back extraction buffer for genomic DNA isolation. After back extraction I saved the viscous pellet, I have seen people save it for protein isolation but was not able to find a protocol to proceed. Can anyone help me with this?
No personal experience of 3-from-1 extraction, but this article seems like the right direction:
Article Isolation and solubilization of proteins after TRIZOL® extra...
They have conducted a pretty thorough comparison of solubilization tactics & solvent comparisons. See CONCLUSIONS for streamlined answer to your question (what they found to be the best overall approach).
Hope this helps!
There are a few kits that allow you to isolate RNA, DNA and proteins from the same sample. I have tested Quick DNA/RNA mini prep from Zymo after binding the RNA to the column you can precipitate the protein content with cold acetone. Check this protocol:
https://files.zymoresearch.com/protocols/_d7003t_d7003_quick-dna-rna_miniprep_plus_kit.pdf
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