Hello
How to extract DNA from water samples without using filter to perform metagenomics?
That depends on what you are trying to achieve...
The logic for filtration is to concentrate microorganisms (and their DNA/RNA) from a large volume of water to a low volume for DNA/RNA extraction. You could potentially use another method of concentrating, e.g. flocculation, but it's likely to be much less effective for low abundance of microorganisms
Most commonly used methods to capture eDNA from aquatic environments. Filtration requires passage of water samples through a filter to trap the DNA whereas the precipitation method uses ethanol to precipitate nucleic acids in the water sample
Extracting DNA from water samples can be a bit more challenging than extracting DNA from other biological samples, such as tissues or blood, because the amount of DNA present in water is often very low. Here's a general overview of the steps involved in extracting DNA from a water sample:
Keep in mind that the specific protocol for extracting DNA from a water sample can vary depending on the type of water sample, the type of DNA you are trying to extract, and the downstream application for which the DNA will be used. It's important to follow a validated protocol and to be mindful of potential sources of contamination throughout the extraction process.
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