How to extract DNA from Eimeria tenella cyst to get high yields?

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Rajul Tomar

It can be done with the help of liquid nitrogen, by freezing the oocysts/sporocysts and then crushing it in a mortar-pestle. ( For high yields alternate cycles of freeze-thawing will be helpful) Also you can use acid-washed glass beads to break the oocysts and then RNAse and Proteinase K treatment DNA can be extracted using chloroform:phenol extraction protocol.

Hudson Alves Pinto

Dear Mona,

I think the protocols described by Zhao et al (2001) can help you in extraction of DNA of E. tenella oocysts.

Best regards,

Hudson

Ali Raza

You can either use the DNA extraction columns or Trizole Extraction method after homogenization of samples by adding glass beads and put in bead beater.