200 g fresh fruit puree is extracted with 200 ml of solvent on orbital shaker then filtration and desolvinization in rotary evaporator to obtain dry extract of 19 gram. Next 0.5 g extract was dissolved in 10 ml to obtain the concentration of 0.05g/ml. Next o.5 ml of the above diluted extract was used in FCR assay. Abs = 0.088
Caliberation equation is 0.0038x+0.0172(Range 20-200 mg/L)
calculation:
(0.088-00172)/0.0038 = 18.63 mg GAE/L.
Now for one gram it would be 0.186 mg GAE/g
Now what will be the unit express with 0.186 mg, either it is per gram extract, per gram sample or per gram dry weight of extract.
What will be the result on dry matter basis. If dry matter content of fruit is 17.5%
Please rectify if wrong.
Dr.Mohammad,
If you'd like to expressed the results in milligram gallic acid equivalents (GAE) per gram of dried weight (DW) of plant material (mg GAE/ g DW). Calculated The content of total phenolics in plant extracts using the following formula:
C=(c*V)/m
where: C is the content of the total phenolics, c is the concentration of gallic acid established from the calibration curve, V is the volume of the extract, and m is the weight of the dried powder plant.
Thanks Abdulelah Ahmad
But, Per gram of dried weight of plant material is not similar to per gram dry extract. It is advised to exclude the moisture content in final calculation to obtain per gram Dw. Because i used fresh fruit for extraction not powder form.
Dr Mohammad
As you mentioned, you have did your work in fresh fruit sample and then extraction process followed.
1. You can express your result only in Fresh weight or extracted weight if you have did your work...(as you described)
2. You can repeat this process with slight modification as measure dry weight of 200 gm fruit and then use this dried sample for extraction of phenolic compounds.
Follow further process and express results on dry weight basis in mg GAE/gm DW.
Hi,
X gram of plant was extracted by amount of solvent. After completely remove of solvent, the obtained extract was dried to reach the stable weight. So we have dry extract. The amount of total phenolic compounds was determined in 1 gram of dried extract by folin- ciocalteu method and based on gallic acid standard curve. By this way we can express the results of total phenolic content as mg gallic acid/ g dry extract.
Thanks Paria
When in a research paper expressed the total phenolic contents as mgGAE/g DW.
My confusion is that either they mean the dry weight of extract or dry weight of plant initially used for extraction.
Moreover, if i aim to convert results from mgGAE/g dry extract to mgGAE/g dry matter then how.
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