My gallic acid curve is in the range of 50-250mg/L. Curve eqn is 0.0034x-0.0725 and abs value is 0.635. 1gram sample dissolved in 20ml from which 1ml was used in the TPC assay.
200 g fresh fruit puree is extracted with 200 ml of solvent on orbital shaker then filtration and desolvinization in rotary evaporator to obtain dry extract of 19 gram. Next 0.5 g extract was...
31 December 2018 7,887 5 View
We have recently extracted the grape seeds with solvent mixture composed of acetone:water:acetic acid(90:9.5:0.5). Which solvent will be used or appropriate to make different dilutions of...
11 December 2018 8,621 6 View
Hello we are working with shimadazu 1700 spectrophotometer compatible with PC. When i set the scan range from 400 to 900 nm for lambda max, after completion of the scan the raw data from 700 to...
11 December 2018 6,397 2 View
Most scientific papers have stated the formula for extraction yield calculation like this, The yield of extract (extractable components) expressed on dry weight basis of pulp was calculated...
05 June 2018 10,362 8 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...
30 July 2024 557 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.
29 July 2024 6,115 0 View
I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.
23 July 2024 8,075 3 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View