How to exprees the results of total phenolic contents as mg GAE/g dry weieght?

200 g fresh fruit puree is extracted with 200 ml of solvent on orbital shaker then filtration and desolvinization in rotary evaporator to obtain dry extract of 19 gram. Next 0.5 g extract was...

31 December 2018 7,686 5 View

Which solvent is apt for making different dillutions of crude extract of grape seeds, if the extraction was performed in acetone:water:acetic acid?

We have recently extracted the grape seeds with solvent mixture composed of acetone:water:acetic acid(90:9.5:0.5). Which solvent will be used or appropriate to make different dilutions of...

11 December 2018 8,506 6 View

Why my specrtophotometer does not show complete raw data of the desired scan range?

Hello we are working with shimadazu 1700 spectrophotometer compatible with PC. When i set the scan range from 400 to 900 nm for lambda max, after completion of the scan the raw data from 700 to...

11 December 2018 6,275 2 View

How to calculate extraction yield?

Most scientific papers have stated the formula for extraction yield calculation like this, The yield of extract (extractable components) expressed on dry weight basis of pulp was calculated...

05 June 2018 9,623 8 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View