How to avoid polypyrrole deposit on counter electrode?

Hi all, Currently, I electrodeposited polypyrrole on carbon electrode but I got some problem when polypyrrole also deposited on the surface of the counter electrode. The concentration of pyrrole...

05 February 2021 2,103 3 View

How to Extract DNA from dried rhizomes, wood, galls etc. stored for a long time?

Dear Respectable Scientists, I have been trying to extract DNA from above mentioned sources using different CTAB/SDS Buffer Compositions and purifying agents but I am not able to extract DNA from...

12 January 2021 5,899 8 View

How to carry out Fisher's Exact Test in SPSS for multiple Response Dataset?

I have a dataset including Categorical variables and involves multiple response variable. 20% of the cells in cross tab has less than 5 frequencies and hence I cannot carry out a Chi-square Test....

26 December 2020 4,180 3 View

**CLOSED** Behavioral changes due to Covid-19?

Hello fellow researchers around the globe :) Happy Holidays!! We are conducting a survey on understanding how device usage during everyday life has developed amid the Covid-19...

21 December 2020 5,363 4 View

Does the Chinese navigation satellite system (Beidou) become global or still regional?

I know that this system was regional before, thus covering specific areas not alla the global

09 December 2020 3,343 3 View

Do the exams for students in electronic education have the same credibility in terms of measuring and evaluating learning as traditional education?

Educators around the world face a new challenge of how to conduct exams to assess what students have learned after the shift towards e-learning following the closure of nearly all universities...

21 September 2020 4,180 15 View

What Features Should an Online Exam System Include?

ith the development of Internet technology, online examination has become more Polar because it helps people save a great deal of energy and time. As an effective and efficient method of teaching...

21 September 2020 2,814 12 View

How can heat transfer coefficient on shell side of Shell and tube heat exchange can be calculated using Ansys Fluent?

I have to calculate heat transfer co-efficient on shell side and plot its variation along the length of heat exchanger

07 September 2020 9,078 6 View

How to get rid of negative Pressure? and my pressure readings are not accurate what can be the cause?

I am simulating shell and tube heat ex-changer i have mass flow inlet and pressure oulet (zero gauge) but my pressure readings are not accurate i have tried velocity inlet but results are same....

07 September 2020 7,298 11 View

AST result in spss ?

hello dear microbiologist Am kinda new and still learning SPSS I want to know what kind of tests should I run for AST results I see lots of paper use chi square. And can anyone provide me with...

10 August 2020 9,911 4 View

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02 March 2021 3,060 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

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28 February 2021 9,936 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View