Suitable materials for orifice plate?

Does the construction materials have any influence on accuracy of orifice flow meter? ( Working fluid: air)

11 October 2018 2,213 2 View

What is the size of the genomic DNA when you extract DNA from Saccharomyces cerevisiae using Zymolyase method ?

Size of the Genomic DNA through Zymolyase method of yeast DNA preparation..

26 January 2017 1,672 4 View

Which Is the f in L^{1}(\mb{R}) with Fourier transorm in L^{1}(\mb{R}), but niether f nor its Fourier transform is in the Wiener's space?

11 February 2016 6,093 1 View

Is aryl boronic pinacol ester stable to basic conditions?

I have an aryl boronic pinacol ester (Ar-Bpin) which has a phenolic -OH group. I would like to use K2CO3 conditions, as well as NaOH conditions (separate reactions). Question is whether Ar-Bpin...

23 October 2013 8,139 2 View

Click chemistry on multiple azide groups - can anyone help?

I am trying to do a small scale (approximately 20 micromol of azide and 50 micromol of alkyne) click reaction using CuSO4.5H2O (5-10 mol%) and (+) sodium -L-ascorbate (20 mol%). My alkyne and the...

17 July 2013 2,480 31 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View