Analyzing genome sequence data. Servers? Softwares?

More Maheswaran Sivakumar's questions See All

I am trying to simulate sputtering from a cylindrical target using RSD software. Can anyone help in creating racetrack for cylindrical target?

The target type is titanium; the height is 900 mm, the width is 70 mm, the race track height is 750 mm and the width is 50 mm.

04 June 2024 1,025 1 View

I have published a paper in a Scopus Indexed Journal recently. When i will be getting DOI ? Is DOI is essential ?

I have recently published a paper in a Scopus-indexed journal. Is DOI essential? How long will it take to get DOI ? Thanks in advance

08 April 2024 4,092 3 View

How to analyze product" Movie Tickets" in SriLankan under the theory of consumer behavior?

In Srilanka we have several movie theatre those to charge price for movie tickets but how to analyse this product under theory of consumer behaviour

20 October 2023 4,263 0 View

How to prepare 500micromole of H2O2 from 6% hydrogen peroxide? ascorbic acid or hydrogen peroxide which better as agents?

For clevage assay

08 March 2023 3,933 2 View

Do lactic acid bacteria produce catalase or hydrogen peroxide?

In catalase test, we add H2O2 to test the presence of catalase enzyme.If the bubbles do not appear, It confirms that it is a lactic acid bacteria. But to test antimicrobial activity by...

19 February 2023 549 4 View

How can I confirm the antagonistic activity of the lactic acid bacteria is not by lactic acid and other organic acids and by bacteriocins?

I need to confirm that the antibacterial activity of the lactic acid bacterial strains is by bacteriocins(the antibacterial peptides). Are there any biochemical tests to confirm it?

14 February 2023 4,859 3 View

How to convert heat flux data of unit W/m2 to Degree celsius per day?

Hi all ! I have daily heat flux data (shortwave, longwave, sensible and latent) in the unit of W/m2. I want to convert them to °C/day. Any leads on how to go about the conversion ? TIA

16 November 2022 4,196 1 View

How to compare the results by using statistical analysis?

The following details are explaining my experimental design and my doubts about the statistical analysis. Plant is growing in Nutrient 1 and Nutrient 2 . The toxicants at difference...

03 May 2022 5,789 3 View

How to fix yeast cells with formaldehyde for tracking of a GFP tagged membrane protein?

Hello everyone, I'm here to seek suggestions for fixing of yeast cells with formaldehyde, which are later to be visualized under microscope for tracking of a membrane protein tagged with GFP. I...

15 September 2021 2,381 2 View

What are the best drought indices to compare?

Dear all, I'm working on drought indices comparison. Can you please suggest an insight into the drought indices that are comparable? Is there any prerequisite to be taken into consideration?

18 August 2021 8,469 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Why is nonpoint source pollution potentially more harmful and difference between point and nonpoint sources of water pollution?

01 August 2024 1,180 2 View

How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?

01 August 2024 7,061 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

How does nonpoint source pollution affect ocean environment & why point source pollution is often easier to manage than nonpoint source pollution?

How does nonpoint source pollution affect the ocean environment and why point source pollution is often easier to manage than nonpoint source pollution?

01 August 2024 8,014 2 View

Arindam Deb

GALAXY is well known and well recognised server to analyse NGS data. You can try this.

Pallavi Gaur

It would be better if you could be more elaborative. If it is a NGS data then you may follow some popular pipelines. If you want to use an easy GUI based environment to analyse NGS data then GALAXY would be best for you.

https://usegalaxy.org/

Maheswaran Sivakumar

Yup. it is thru NGS. Thanks for the reply. will ask you if have any doubts.

Thanks all of you.