I have received the RNAseq data outcomes as alternative splicing and differentially alternative splicing in excel files
For Alternative splicing, there are data for each sample as the following
· Alternative 5' Splicing Site(A5SS)
· Alternative 3' Splicing Site (A3SS)
· Mutually exclusive exons (MXE)
· Retained Intron (RI)
· Skipped Exon (SE)
And this is the detail of Retained Intron (RI) this is excel file format as in photo 1
For differential Alternative splicing, there are data for each group (control and test) as the following
· Alternative 5' Splicing Site (A5SS)
· Alternative 3' Splicing Site (A3SS)
· Mutually exclusive exons (MXE)
· Retained Intron (RI)
· Skipped Exon (SE)
And this is the detail of Retained Intron (RI) ) this is excel file format as in photo 2
Abbreviation in photo1,2
SC: The number of reads across the skipping junction, repeated samples are separated by semicolons
IC: The number of reads across the inclusion junction, repeated samples are separated by semicolons
PLEASE I need suggestions from the expertise of RNAseq data interested scientists, On how to estimate IR from that data that I have?