How to enrich a mixed sample in Prokaryotic RNA ?

François-Étienne Sylvain @Francois-Etienne-Sylvain

25 May 2020 0 1K Report

I am currently working on the transcriptome of Prokaryotic communities of fish gills. Whole fish gills were used for RNA extractions wth Trizol. After a test sequencing run, > 99% of the sequences obtained were Eukaryotic (host) RNA sequences. Does somebody have any approach to suggest to enrich our samples in Prokaryotic RNA?

Thank you very much for your help!

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