How to elute a protein band of interest from Native PAGE ??

Why I can't see any band in SDS-PAGE?

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How to preform densitometry on SDS-page bands?

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Low-yield gel extraction problem?

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Transfection in HEK293T cells?

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Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

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How to retain the a GFP tagged gene expression in stable cell line?

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What is the relationship between protein structure and N or C terminal tagging choosing?

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Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

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How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

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Adesola Julius Tola

Hi Kumar,

I think for you to achieve this all depends on what you want to use the protein for?This can be done by cutting the protein band of interest and use sonication method.You can follow the protocol attached below:-

I hope this will help you.

All the best!

Dominique Liger

Hi there,

I guess you intend to recover the protein of interest under its native conformation. Electroelution is therefore applicable.