How to elute a protein band of interest from Native PAGE ??

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Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

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Are protein samples in SDS compatible with ELISA?

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How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

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Adesola Julius Tola

Hi Kumar,

I think for you to achieve this all depends on what you want to use the protein for?This can be done by cutting the protein band of interest and use sonication method.You can follow the protocol attached below:-

I hope this will help you.

All the best!

Dominique Liger

Hi there,

I guess you intend to recover the protein of interest under its native conformation. Electroelution is therefore applicable.