We used to dilute the DMSO containing solution with water and pass the mixture through C18 SPE cartridge.The compound should bind to the C18 material, so after loading you can wash it with water and then elute with organic solvent.

DMSO is miscible with water while EA and DCM are not. I've precipitated products by adding water to the DMSO solution then filtering with a water wash. Extraction will work, but it may take several iterations and you may never completely remove the DMSO as it is miscible with both of the solvents you list.

If you have a C18 SPE cartridge (or small C18 flash column- 5g size), you can mix some water with the DMSO/sample mix and load it onto a conditioned cartridge. Wash with water, then wash with ethyl acetate. The ethyl acetate won't harm a silica-based C18 SPE or flash column.

my organic compound which is carboxylic acid cannot be seen by UV

I need to perform TLC

Water washing will be a good way ot eliminate DMSO

I would suggest freeze-drying.

As Liu Paul said,extraction with other solvents like chloroform or DCM and then water washing is a good choice to eliminate DMSO. Also, if the products are stable enough, a high vaccum with a oil pump at 40 to 50 degree maybe work well.You can have a try.

Either dry freezing to competely remove or addtion of water than extraction with DCM is the other way.

Add ethyl acetate or DCM to the organic reaction mixture, then add calcium chloride (anhydrous). Calcium chloride associate DMSO. Then filter.

CaCl2 + 6DMSO = CaCl2*6DMSO

Is this really work. I doubt about this since one have to check the nature of molecules acidic or basic he want to separate from DMSO.

If organic molecules are acid phobic, that add anhydrous sodium sulphate (less effective than calcium chloride)

To get rid of DMSO with an extractive workup, I would first evaporate most of it (you will need a good vacuum pump to keep the temperature reasonable), add the desired solvent and water, separate phases, and wash the organic phase at least one more time with water or brine.

Bromocresol green stain is selective for acidic groups in TLC, and your carboxylic acids will likely stain with phosphomolybdicacid or cerium ammonium molybdate ("general stains") as well.

the best one I've found is since many years is mixing with an enough amount of water and then extraction with DCM followed by washing the organic layer, two to three times, with water and then drying with either anhyd, Na2SO4 or MgSO4. Evaporation of solvent will provide you with the sample completely free of DMSO.

I agree with Shahid Ameen very much.Because I have succeed in get rid of DMSO from my samples.

You can used a dialysis membrane first to remove unreacted solvent.then freeze dry your sample.

I have eliminated DMSO from my samples by adding much water, extracting with diethyl ether at least three times, waching the combined organic layers with water, drying with Na2SO4 or MgSO4 and evaporating the solvent.

Dr.M.S.Chandel from Artek Surfin Chemicals Ltd. Mumbai , India

I agree with Shahid Ameen very much.Because I have succeed in get rid of DMSO from my samples but water washing required 7-8 times

Add water then transfer to rota-vapor to be sure you haven't any organic then transfer to lyophilizer it will dry your compound completely in a safe way and you will not lose anything of your compound.

Better to do extrative work up with diethyl ether or MTBE. solubility of DMSO is low in these solvent in comparison to water. rememer to wash combined organic layer with water later.

first take reaction mixture in ethyl acetate. if you have used 1 ml DMSO, add more than 10 ml of water during work-up. give repeated water wash.

take the sample in more than 10 vol water and extract with TBME, DMSO will not be generally come in ether layer.....

I think, mixing with water (10 times) and then extracting with MDC and drying the MDC layer, will help you....

Very thankful to all of you for all your answers.

You can use Nitrogen, rotary evaporator or you can use vaccum

If your product desolves little in water, you may add water directly to your crude and filtrate...

The best way to remove DMSO is by freezer dryer your sample with H2O (H2O:DMSO 10:1) you can't remove DMSO completely by washing, extraction or by rotavap.

Washing with water can extract the DMSO reaction medium

Lyophilisation is very efficient

When it works right, a Biotage V-10 evaporator can dry down DMSO. But when it is working properly.

You can use speed-back system. It is a vacum system with a centrifuge incorporated, in this way it is possible to dry efficiently DMSO, DMF or water

Rota-vaporization concomitant with phase separation technique would certainly be helpful.

All the responses to the question from Sandipan Jana are quite convincing.

you have to dilute DMSO with water and extract the organic material with chloroform. and then wash the chloroform multiple times with water (3 or 4 times).

We used to dilute the DMSO containing solution with water and pass the mixture through C18 SPE cartridge.The compound should bind to the C18 material, so after loading you can wash it with water and then elute with organic solvent.

Dilute the reaction mixture with cold water and extract it either with ethyl acetate or methyl tertiarybutyl ether and wash the organic phase with excess cold water 3-4 times

Multiple washing with water followed by lyophilization (freeze drying) is one of the best option to elimate DMSO perfectly from the organic mixture in order to do GC and make the solvent as ethyl acetate or DCM. In my opinion, ethyl acetate will be better as compared with DCM.

in our center dilute DMSO with water and  seperate the organic material with chloroform or ethyl acetate,  it seems  that ethyl acetate is superior  as compared with chloroform.

I agree with Sanjay Mishra ji to go for multiple washing with water followed by lyophilization. It will definitely improve the yield and purity of the compound as well, followed by extraction, preferably, with ethyl acetate 

Hi! I have a similar problem where I need an alternate for rota vapor in order to separate DMSO from my organic compound mixture. I can't use the water method also since my compound is soluble in water! Help me!

I am facing the same problem like Anu Maashaa Nedumaran