e.g for protein standard curve we use BSA standard curve to get the estimation of crude unknown protein.If any one have any protocol regarding this issue.Kindly share the link or pdf.
You can check the materials and methods section of the paper. Here is the link to access the paper.
https://www.researchgate.net/publication/233579090_Increased_enantioselectivity_of_lipase_in_the_transesterification_of_DL--3-phenyllactic_acid_in_ionic_liquids
Article Increased enantioselectivity of lipase in the transesterific...
9/21/17
Dear Khalid,
The calibration curve is produced in the same way as for the protein assay. In this case, the PNPA is broken down by lipase into palmitic acid and para-nitrophenol (pNP). You prepare a calibration curve by plotting uG (or uMole) pNP vs. Abs @ 410 nm. For your assay, measure the A410 from the liberated pNP of your samples, then determine the amount of pNP released/min by reference to your std curve. From this, you can calculate the # of uMoles of PNPA hydrolyzed/min by the lipase.
I hope this information helps you.
Bill Colonna
Center for Crops Utilization Research, Iowa State University, Ames, IA USA
Just for the record, there is a good chapter in the book Pseudomonas Methods and Protocols:
Article Determination of Lipolytic Enzyme Activities
- Badges
- Science topic
- Similar topics
- Microbiology
- Assays