How to draw a standard curve for lipase assay using PNPA as substrate?

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How to draw a standard curve for lipase assay using PNPA as substrate?

e.g for protein standard curve we use BSA standard curve to get the estimation of crude unknown protein.If any one have any protocol regarding this issue.Kindly share the link or pdf.

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Abhishek Sharma Popular answer

Make standard of Para nitro phenol (micromole or microgram) as

Lipase breaks pNPA to pNP and Acetic acid. Observe reading at 410.

Use winker and Stuckmann Assay.

Kapil Kumar

may be this link will behelp full to you...

https://www.youtube.com/watch?v=eXMvcLroUbU&t=1s

Khalid Mehmood Wani

Thank you kapil

Abhishek Sharma