Luminescence assay for VEGFR-1 dimerization background signal went up?

I am working on a luminescence assay (one of the Glo-kits from Promega) to detect VEGFR-1 dimerization due to PlGF-2 binding. The background signal (control without ligand binding) suddenly went...

09 October 2018 5,365 0 View

PEI Max transfection: why did my cells die and media turn pink?

I did a PEI transfection in 6-well plate (non-binding), 2mL of CHO suspension cells at 1M/mL. The transfection was stopped after 3 hours. I checked the cells the next day with FACS and microscope...

03 April 2018 8,539 3 View

What blood glucose level is considered diabetic in mice?

06 July 2016 9,616 6 View

Is statistical analysis performed differently when using samples from each individual animal or samples pooled from several animals?

We pool samples from 3-5 animals and is stat analysis done differently comparing to testing samples from each separate animal?

31 December 2015 7,352 8 View

How can I get rid of air bubbles trapped under coverslip for 8 chamber slide?

We culture cells in 8 chamber slides and do immunofluorescent staining. We put one drop of mounting media on each of the 8 wells and drop the coverslip at a 45 degree angle. Does anyone have tips...

31 December 2015 9,786 4 View

How is glucose concentration in beta-cell (islet) culture chosen?

11 December 2015 4,244 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View