Helen Wong

7 Questions 1 Answers 0 Followers

Questions related from Helen Wong

Luminescence assay for VEGFR-1 dimerization background signal went up?

I am working on a luminescence assay (one of the Glo-kits from Promega) to detect VEGFR-1 dimerization due to PlGF-2 binding. The background signal (control without ligand binding) suddenly went...

10 October 2018 5,395 0 View

PEI Max transfection: why did my cells die and media turn pink?

I did a PEI transfection in 6-well plate (non-binding), 2mL of CHO suspension cells at 1M/mL. The transfection was stopped after 3 hours. I checked the cells the next day with FACS and microscope...

04 April 2018 8,572 3 View

What blood glucose level is considered diabetic in mice?

I was looking into different literature and I want to know if there is a guideline for fasting glucose level for OGTT glucose level. It seems like mice with >8mM (fasting) and >7.8mM (OGTT)...

07 July 2016 9,664 6 View

How can I get rid of air bubbles trapped under coverslip for 8 chamber slide?

We culture cells in 8 chamber slides and do immunofluorescent staining. We put one drop of mounting media on each of the 8 wells and drop the coverslip at a 45 degree angle. Does anyone have tips...

01 January 2016 9,820 4 View

Is statistical analysis performed differently when using samples from each individual animal or samples pooled from several animals?

We pool samples from 3-5 animals and is stat analysis done differently comparing to testing samples from each separate animal?

01 January 2016 7,387 8 View

How to do statistics for studies used pooled animal samples?

stats

01 January 2016 8,716 1 View

How is glucose concentration in beta-cell (islet) culture chosen?

I'm wondering why we normally use 11.1mM (human) and 16.5mM (mouse) glucose in islet culture.

12 December 2015 4,276 4 View