You can use multiplex fluorescence in situ hybridization (M-FISH) to detect marker chromosomes in prenatal diagnosis

Yes I know Angela,but I have not M FISH, I will do Fish with different LSI probes in one slide

any way thank you

Dear Luis,

I imagine you have already solved your problem since one year has passed!! Sorry to see that so late!

But sequential FISH is quite simple and robust. You just have to hybridize your first probe, then you remove your first probes under denaturing conditions (70% formamide-2SSC), dehydrate your sample, and rehybridize it with the new probes. I can give you a more detailed protocol, if you're still interested.

I did sequential FISH by other way, but your protocol will be very useful to me

thank you very much. I did the hybridation with diferent probes at the same time and i get very good results

luis

Dear Elodie,

I am interested in doing sequential FISH. What protocol did you use?

Thanks in advance, 

Itu