Is there any available online tool, which can be used to do primer-check for primer location in terms of Exon/Intron binding sites? Similar In silico website, which is currently not working is "projects.insilico.us /SpliceCenter/PrimerCheck.jsp". Is there any online alternative?
Hi Mustafa
why don't you use the UCSC database (https://genome.ucsc.edu/) where there is a tool called "in silicon PCR"? you can thereafter see where your primers are mapping.
all the best
fred
Frederic Lepretre However is it possible for this website (https://genome.ucsc.edu/) to show primers' location at Introns/Exons binding site? I am trying to fulfill the MIQE guidelines of minimum information for publication of quantitative real-time PCR experiments.
yes, if you apply your primers to the in silica PCR tool, you'll be directed to the results and possibility to assess their locations. if you don't have your own primers and don't have the possibility to design them, upon hg19 genome, you can show the optional track named qPCR primers.
fred
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