I am planning an experiment of determination of Polysorbate 20 and 80 from my protein samples. How to I proceed with the sample preparation for LCMS ? Looking forward to your opinion.
You need an SEC column. However, each peak will be the sorbate moiety or its daughters. You will not be able to quantitate the amount of 20 or 80 or proportion. What is your sample matrix?
This morning I thought to screen my "data dungeons" for Polysorbate 80 and Tween 80 and found the attached documents for you. Together with my teams, we used this information to analyze samples from a different origin. Just a comment: Eluents and applications developed for Corona CAD detection are compatible with MS-detection. I hope the attached documents hold interesting information.