How to evaluate motivation in scholars, teachers and students who work from home during pandemic?

Dear all, What tool can be used to measure motivation in scholars, teachers and students who are in home isolation or work remotely? Any specific tool suitable for all people involved in Academia...

08 December 2020 7,968 10 View

How to replace the liquid blocker?

Dear colleagues, In my immunofluorescence stainigs I use liquid blocker pen, before solution with 0.3% Triton X-100. This helps to avoid dripping of solutions with primary antibodies and Triton...

30 September 2020 9,134 4 View

No title

06 July 2020 4,133 2 View

How long can one store primers?

Dear colleagues, one year ago we put our liquid primers in a high concentration into a -80 fridge. Can we still use them for the qPCR?

11 May 2020 4,521 16 View

What is an acceptable small sample size to carry out principal component analysis for a rating scale with 16 items???

Hi Can someone tell me what would be an acceptable sample size for principal component analysis for a rating scale with 16 items. Thanks!

30 March 2020 9,756 3 View

How to insert image in introductory text of survey in Opinio?

Hi I need some technical help. I am trying to insert an image in the introduction of a survey in Opinio. I click on 'insert image' button, then I am prompted to paste the url of the desired image,...

28 March 2020 1,543 3 View

How does the Excel work with GRG algorithm?

I'm struggling with my qualification work. Excel methods solve the math problem easy, but I don't catch the idea how he does it. I need to understand it to create the code or just to describe the...

12 March 2020 6,567 6 View

What to do if Fatal error: Atomtype LC3 not found and force field is fine?

When I was simulating my protein, "Fatal error: Atomtype LC3 not found" had occured. I checked my force field atom type and it contains LC3 with correct parameters. Before this error I used...

10 March 2020 2,189 2 View

Has anyone optimized ViromerRED in THP-1, H9, and MCF7 cell lines?

I am currently attempting to optimize ViromerRED transfection in THP-1, H9, and MCF7 cells. Our initial transfections with the first batch was promising, but the new viromer we received doesn't...

15 October 2019 5,053 4 View

Can anybody explain why does the line on gel disappear after 15 cycles of PCR?

We make test PCR to choose optimal quantity of cycles and amount of cDNA for amplification, and there are lines on 9 cycles and 12 cycles (same sample), but the line on 15 cycles disappear (also...

02 April 2019 3,158 7 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View