Dear RG colleagues,
Can you guys please give me some tips to dissolve Devazepide (CCKA(1) Receptor inhibitor)) to inject safely with correct dose to mice?
I saw that it was well dissolved to DMSO but when I further add PBS, it become hazy.
Adding Tween 20, 80 or PEG my increase the solubility of Devazepide but they are not yet available in our lab and also concern about the safety and side effect for the mice.
Thank you very much in advance for your kind help!
Best,
Trung
Dear Trung Trần Quang thanky for asking this interesting question. There are various articles available in which the use of Devazepide for injection has been described. For example, a typical procedure is described in the article cited below. It is stated there that "Devazepide was dissolved in 50 µl of dimethyl sulfoxide and 50 µl of Tween 80, and further diluted in saline."
Synergistic Interaction between Leptin and Cholecystokinin to Reduce Short-Term FoodIntake in Lean Mice
https://www.jstor.org/stable/43228?seq=1#metadata_info_tab_contents
In another article it is mentioned that "Devazepide was dissolved in DMSO and saline."
Antipsychotic Potential of CCK-Based Treatments: An Assessment Using the Prepulse Inhibition Model of Psychosis
https://www.nature.com/articles/1395224
I hope this helps. Good luck with your research and best wishes!
Dear Prof Frank T. Edelmann , thank you very much for your kind and promt answer.
Actually I have gone throught those nice papers but I have still some concerning points:
- For the former, they did not mention how much Devazepide should be used with those 50µl DMSO and Tween 80. Moreover, we have only Tween 20 now and these Tween are not sterilized for injection
- For the later, they did not indicated clearly how much amount of Devazepide, DMSO and saline. I tried week DMSO and saw that the solubility is upto 100nM. But when I add mor PBS or saline to avoid unexpected effect of DMSO when injecting to mice, the Devazepide solution become hazy (not homgeneous but lots of visible white substance like crystals inside). For that, I am afraid that the dose will be calculated not correctly and it may harm the mice in some way and may bias the inhibitory effect.
If you have any other tips or experiences, please teach me more!
Gratefully,
Quang Trung Tran
Dear Trung Trần Quang many thanks for your kind response and explanation. I do not have any personal experiences as we are inorganic chemists. However, the published procedures apparently vary significantly. In the article cited below it is stated that
"Devazepide was dissolved as a 1 mg mL–1 stock solution in a 7:3 (v v–1) mixture of PEG 400 and glycerine. The stock solution was stored at 4 °C and was
diluted in 0.5 mL of saline prior to injection."
At least the authors here specify the exact amounts.
PEGylated cholecystokinin is more potent in inducing anorexia than conditioned taste aversion in rats
Article PEGylated cholecystokinin is more potent in inducing anorexi...
In the study cited below the devazepide was use as suspension:
CCK-A receptor antagonists have selective effects on nutrient-induced food intake suppression in rats
Article CCK-A receptor antagonists have selective effects on nutrien...