What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

How to calculate ECC using HFSS software?

Good morning. Now i am designing MIMO antenna for Ultraband Applications. Can anyone suggest how to plot ECC (Envelope correlation Coefficient) Using HFSS 15 software. Thanks in advance

13 February 2020 5,261 6 View

What is the zeta potential value of deionised or distilled water?

I am getting zeta potential value of about -30 mV for distilled water obtained from a commercial supplier. What it means? What should be the zeta potential value of distilled water? How can I...

10 October 2017 2,791 19 View

Zeta potential for amine functionalized Nanoparticles?

What will be the sign of zeta potential for amine functionalized nanoparticles? The amines are neutral (not protonated). Due to the electron rich character of amine functional group shall I expect...

30 July 2016 9,866 4 View

How to compare more than 50 groups?

i want compare more than 50 groups? SPSS could not performing ANOVA. which test shal i use to compare these groups ? kindly give your suggestions

05 July 2016 3,389 6 View

What is the statistical methods or test to identify first five best groups from several groups?

I am doing some diversity analysis for mineral content. If i want to compare and identify five best groups/samples from 70 samples (for 10 different parameters-independent variables), which...

30 May 2016 956 3 View

Is the zeta potential value valid for amino acid functionalized nanoparticles?

I have modified my carbon nanoparticle surface with amino acids. Now I would have both amine and acid functionalities on the surface of my nanoparticle. The measured zeta potential value is about...

31 December 2015 6,592 10 View

Can anyone suggest literature on Regional Innovation Systems in solar energy in the Indian context?

26 March 2015 5,749 2 View

What can be used as a standard for alpha amylase and glucosidase activity?

Why we should use acarbose as reference for alpha glucosidase activity? Is there any other standard that can be used for this enzyme assay? Can maltose be used as reference for alpha amylase activity?

07 October 2014 1,297 1 View

DTT or BME - which one is better for RNA isolation from 293T cells?

In a Qiagen RNA isolation kit either DTT or BME has to be added to the RLT buffer.

26 March 2014 4,998 4 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

What is the composition of the solid and broth agar to grow Streptomyces from freezed sample? what are extraction steps of products from broth agar?

Please what are the composition of the solid agar could I use to grow the bacteria? what are the composition of the broth agar could I use to grow the bacteria and how to extract the natural...

26 January 2021 5,006 1 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View