How to dissolve and sterilize L-Tryptophan for IAA colorimetric assay ?

01 January 1970 1 10K Report

Dear all,

I want to know please :

1)- How to dissolve L-Tryptophan since it is unsoluble in ethanol. Sme suggested me to use NaOH, would you please telle me what concentration of NaOH suould I prepare.

2)- How to sterilize L-Tryptophan solution ? Since it can't be autoclaved at 121°C.

Thank you in advance.

Cheers.

Rajesh Dev Sarkar

You can dissolve it in water and for sterilization you can use 0.2 micron filter sterilizer.

Badges
Science topic

Similar topics
Microbiology
Assays

More Wiem Alloun's questions See All

Does anyone have the standard curve of IAA, Please ?

Dear All, I would like if someone can send me a standard curve of IAA (Indol 3 acetic acid) Thank you

06 July 2019 1,942 3 View

How can I stimulate spore production of Actinobacteria in liquid growth media ?

I want to stimulte spore production of an actinobacteria isolate in liquid growth media. Can you help me ?

10 November 2018 2,626 3 View

Where can I perform an HPLC for bioactive compounds please ?

I am searching a lab that has HPLC facility to help me to characterize bioactive compounds which is the next step in my research project.

08 September 2018 3,846 3 View

What's new in Applied Biology ?

Projects and Methods

11 December 2016 1,864 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View