Hi everybody!
I am establishing a protocol for organotypic brain slice culture in my lab. We need it for electrophysiological study and the standard method with growing slices on membrane inserts didn't prove useful, so I opted for the one in which slices are glued to the coverslip using plasma/thrombin clot.
Nevertheless, the pipette used for electrophysiology can't go through the clot and we will need to have it dissolved before the experiment. Does anyone know safe methods to perform it and not influence the slice underneath? Logical solution would be using plasmin, but I am not sure how well it works in vitro and have no possibility to test it without buzing it first.
Thanks a lot!
Marta
Hi Marta Nowakowska .
If I understood your question you need to fix the slice somehow to avoid it floating while patching, right? One way to pin the slices down in your chamber without using a metal harp is making them adhere to a smaller coverslip (usually a round 10 or 12 mm one) pretreated with poly-l lysine before transferring them to your rig. You may find these poly-L lysine-treated coverslip ready for purchasing or treat them yourself.
Hope it helps! Good luck!
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology
- Virology
- Virus