Using a fixed angle rotor with Sucrose gradient /cushion ultracentirfugation?

I am trying to fractionate virus from plasma into different densities by sucrose gradient but we only have fixed angle rotors... Has anyone tried doing that with a fixed angle? Should I use the...

13 January 2021 8,372 3 View

Can I leave blot in block buffer for 48 hours?

Is it ok ti leave the blot in non fat milk for 48 hours in +4 degrees or is it better to leave it tbs-t for that extra 24hrs?

23 February 2020 7,475 2 View

Should the HCV Core protein fused with His-tag (without linker) be extracted in the insoluble or soluble protein fraction?

I have infected insect cells SF9 with recomb. baculovirus expressing Core.His and another with GFP. I have extracted the soluble proteins after 3 days and did western blot using anti-his,...

10 February 2020 2,359 4 View

in concrete application Which more benefit adding Nano Carbon tube or Nano cellulose from economic side and performance?

My dear colleagues, in concrete application which more benefit adding Nano Carbon tube or Nano cellulose from economic side and performance?

19 April 2019 4,501 8 View

What is the best way to of the waste plastic fiber to enhance some of the mechanical properties of concrete?

Dear Colleagues What is the best way to enhancing the shape and the outer surface of the waste plastic fiber to enhance some of the mechanical properties of concrete?

21 September 2018 7,603 1 View

Which is more useful in improving the impact resistance of the concrete. The use of aluminum waste as an alternative to sand or cement?

Dear Colleagues Which is more useful in improving the impact resistance of the concrete. The use of aluminum waste as an alternative to sand or cement?

31 May 2018 5,727 6 View

Polymer diposition on nanoparticles?

I wanted to diposite a polymer on nanoparticles,but the TEM results shows that that the polymer diposite on an aggregated nanopartilces together. is that a problem of the nanoparticles dispertion...

04 April 2017 204 5 View

How I can calculate vertex coordinates from vertex normal in triangular mesh ?

I have triangle mesh and calculate normal of triangles then calculate vertex normal and do some calculations on it and want to calculate vertex coordinates from this vertex normal after do...

01 November 2016 992 3 View

How to measure catalase activity in a plant extract?

can you describe please the contents of a simple reaction to measure catalase activity in a plant extract and is it possible to be a water extract or should it be in phosphate buffer? thanks a lot

05 April 2016 9,175 3 View

Standards in ELISA??

28 February 2016 9,668 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View