How to differentiate Pseudomonas fluoresence and Pseudomonas aeruginosa based on morphological ,cultural and biochemical characteristics
They can be differentiated from their growing temperature.
P. aeruginosa can grow at 42C but P. fluoresence don't grow at more than 30C.
A simple nitrate test can be useful in distinguishing P. aeruginosa (positive) from P. fluorescens (negative).
P. aeruginosa:
is resistant to kanamycin,
can grow at 42°C,
has a complete set of the denitrification enzymes.
You can use a diferential media like kings b or King a P aeruginosa produces a Blue pigmentation meanwhile fluorescens green
Pseudomonas fluoresens and Pseudomonas aeruginosa are both included in the Pseudomonas fluorescent group. The Cetrimide agar is known to be the selective medium used for differentiating and isolation of the Pseudomonas aeruginosa from other pseudomonas species through comparison of their pigment. This medium allows the Pseudomonas aeruginosa to exhibit its characteristics of having blue or blue-green pyocyanin while inhibiting other bacteria. Moreover, Pseudomonas fluoresens can be isolated through King's media that produces fluoresce under UV light. In addition, the Pseudomonas fluoresens grow at 4 °C while the Pseudomonas aeruginosa heightens at 42° C.
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