Dear Monokesh

In order to differentiate MO3.13 cell line into mature oligodendrocytes, you just need to replace FBS from growth media by 100 nM PMA. This protocol generally takes 5-7 days to get maximum expression of mature oligodendrocytes marker such as MBP, MOG etc.

Growth media: DMEM High glucose + 10%FBS (Glutamax and Pen-Strep)

Differentiation induction media: DMEM High Glucose + 100nM PMA (Glutamax and Pen-Strep)