How to develop a HPLC method ? selection of mobile phase and column, any reference guide ?

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Bujji Reddy Kanchi

Dear Mohammad Etman,

Thank you sir.

does column selection depends upon functional group of compound or any other properties of compounds ?

Gunawan Indrayanto

Hi, please open the guideline from Agilent, best regards

https://www.agilent.com/cs/library/eseminars/Public/Microsoft%20PowerPoint%20-%20Rapid%20HPLC%20Method%20Development.pdf

Aditya Trivedi

Dear Bujji

first of all study the solubility profile of your drug candidate

secondly if you want to study combination drugs the select the common solvent for the combination

avoid complex mobile phase