I want to know the schematic methodology to develop a breeding pipeline for soybean pureline cultivar.
What is your actual aim? Breeding and genome sequencing are two different thing.And for breeding pipeline, why should genome sequencing be a part of it?
The following website may be helpful
https://www.soybeanresearchdata.com/Project.aspx?id=52183
sorry am horticulture research in itinerant not breeding
In terms of your soybean breeding the idea would be use the genomic analysis to find critical gene areas and sequences for yield and adaptability You will want to conserve this so identifying them is very important. . Genomic analysis will assure that as say you add resistance genes by backcrossing that resistance genes aare added but at the same time that critical yield areas conferring the genetics of yield and adaptation are not lost but rather are conserved. If you only stress on the disease reaction critical genetic base for yield and adaptability might be lost. No matter the tools employed field yield tests are always the proof in the pudding.
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