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Use the method of incorporation in liquid medium (checkerboard method) on Mueller Hinton broth medium

I suggesting to read this paper (Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteria) 2012

It depends in what the nanoparticles are incorporated (suspension, or in a material?) but the killing effect depends on either silver ions or, if small enough, direct incorporation of nanoparticles into the bacteria. Generally speaking, they do not diffuse through agar like antibiotics so a disc diffusion method would not be suitable. If in an aqueous suspension, you could use a simple doubling dilution method, but the method of test needs to be linked to the intended application: are you intending them to work in suspension, or when incorporated into a (bio)material?

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Use the method in corporation in liquid medium Broth medium

Please use incorprate method in appropriat medium. Be careful. For a good repartition let difuse for 24 hours