I'd like to quantify the total saponin content in food.
Does anyone have any protocol for this?
Waleed Mohamed Abd Elaleem
Total saponin content was determined through spectrophotometry,
as described by Medina-Meza, Aluwi, Saunders, and Ganjyal
(2016). Briefly, 0.25 ml of saponin extract of each sample solution
was added to 1 ml of reagent mixture (glacial acetic acid/sulfuric acid
1:1 v/v). The mixture was vortexed and reacted at 60°C in a water
bath for 30 min and then cooled. The absorbance of the sample
was measured at a wavelength of 527 nm using a spectrophotometer
(Multiskan GO, Thermo Scientific). Oleanolic acid was used as a
standard (0–1,000 μg/ml). Total saponin content was expressed as g
100 g−1 of oleanolic acid equivalents.
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