I have observed the decrement in the immobilized enzyme activity in a specific range of inhibitor. The biosensor response was determined in terms of conductivity change in presence of inhibitor. On increasing the inhibitor concentration from 0.5 mM to 5 mM, the conductivity decreases linearly.
When i plotted the conductance (mS) vs inhibitor concentration (mM), i got a regression line equation y = -7.1636x + 45.182.
When I plotted residual activity (%) vs inhibitor concentration, the regression equation become y = -15.242x + 96.132.
When i plotted Percentage inhibition (%) vs inhibitor conc., the regression eq. become y = 15.459x + 2.7754.
So what will be the sensitivity of the biosensor? From which plot it will come i.e. Conductance vs inhibitor conc. or Percentage inhibiton (%) vs inhibitor conc ??
Dear Nishant,
The sensitivity unit must be mScm-2mM-1 for your system, so the plot you have to use is the first one.
Dear Nishant,
I agree with Burak about sensitivity. Another measurement you might want to consider in addition is to determine the Ki for the enzyme in the biosensors.
Thanks Burak sir and Shelly mam. But what is (2 mM -1 ) in the sensitivity mScm-2 mM - 1. In some papers it is reported that the sensitivity is the slope of regression eq. And in my case the slope of ist plot is 7.16. So 7.16 mS / mM should be the sensitivity. Is this correct?
mS per centimeter squared per mM, so you need to divide your slope by the b the area of the electrode
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